Che Noriah Othman
Senior Lecturer
Title: Chemopreventive study of Clinacanthus nutans on cervical cancer cells and GC-MS analysis
Biography
Biography: Che Noriah Othman
Abstract
Introduction: Chemoprevention is a new approach to suppress, delay or reverse the process of carcinogenesis instead of current treatment that causes many side effects and also possibility of recurrence of cancer. In South East Asia, Clinacanthus nutans Lindau (CN) has been used traditionally as herbal medicine to treat various diseases including cancer. However, the therapeutic potential of CN has not been explored for cancer prevention and treatment.
Objective: This study was conducted to evaluate the chemo-preventive property of CN extract on cervical cancer and to identify the compound in CN extract that has anti-proliferative potential using human cervical adenocarcinoma (HeLa) cell line.
Methodology: 500 gram of dried powdered CN was immersed in ethanol for 3 days. After filtration, the extract was concentrated in vacuo to give crude ethanol extract of CN (EECN). EECN with concentrations of 0.72-1.44mg/ml were treated on HeLa cells to get the IC50 value. The phytochemical contained in the crude extract of EECN were identified using GC-MS.
Result: Results shows that EECN inhibit the growth of cancer cells at IC50 value of 1.08 mg/ml. GCMS analysis of EECN showed that Lupeol, lup-20(29)-en-3-one, β-Amyrin, Linoleic Acid ethyl ester and Squalene were among the identified components of EECN. Lupeol is suggested as the main active compound that might contribute to the chemopreventive property of CN.
Conclusion: These results suggest that EECN exhibits anti-proliferative effect on HeLa cells and potential candidate to be a chemoprevention agent in anticancer drug discovery.